The terms "sense" and "antisense" are relative only to the particular RNA transcript in question, and not to the DNA strand as a whole. In other words, either DNA strand can serve as the sense or antisense strand.
Antisense and RNA interference (RNAi)-mediated gene silencing systems are powerful reverse genetic methods for studying gene function. Most RNAi and antisense experiments used constitutive promoters to drive the expression of RNAi/antisense transgenes; however, several reports showed that constitutive promoters were not expressed in all cell types in cereal plants, suggesting that the
When dsRNAs radiolabeled within either the sense or the antisense strand were incubated with Drosophila lysate in a standard RNAi reaction, 21- to 23-nucleotide RNAs were generated with high efficiency. Single-stranded 32 P-labeled RNA of either the sense or antisense strand was not efficiently converted to 21- to 23-nucleotide products. The
Saliva is a matrix which may act as a vector for pathogen transmission and may serve as a possible proxy for SARS-CoV-2 contagiousness. Therefore, the possibility of detection of intracellular SARS-CoV-2 in saliva by means of fluorescence in situ hybridization is tested, utilizing probes targeting the antisense or sense genomic RNA of SARS-CoV-2. The positiveโ€sense genome is replicated in two stages, initially the positive strand is copied to make a negativeโ€sense RNA, which then functions as the template for transcription of many new positiveโ€sense genomes. Virus infections can be detected at different stages throughout the infection cycle for diagnostic and scientific purposes.
A few DNA sequences in prokaryotes and eukaryotes, and more in plasmids and viruses, blur the distinction between sense and antisense strands by having overlapping genes. In these cases, some DNA sequences do double duty, encoding one protein when read along one strand, and a second protein when read in the opposite direction along the other
์„ผ์Šค ( ์˜์–ด: sense )๋Š” ๋ถ„์ž์ƒ๋ฌผํ•™ ๋ฐ ์œ ์ „ํ•™ ์—์„œ ํ•ต์‚ฐ ๋ถ„์ž, ํŠนํžˆ DNA ๋˜๋Š” RNA ๊ฐ€๋‹ฅ์˜ ์•„๋ฏธ๋…ธ์‚ฐ ์„œ์—ด์„ ์ง€์ •ํ•˜๋Š”๋ฐ ์žˆ์–ด ๊ฐ€๋‹ฅ์˜ ์—ญํ• ๊ณผ ๊ทธ ์ƒ๋ณด์„ฑ ์„ ๋‚˜ํƒ€๋‚ธ๋‹ค. ๋ฌธ๋งฅ์— ๋”ฐ๋ผ ์„ผ์Šค๋Š” ์•ฝ๊ฐ„ ๋‹ค๋ฅธ ์˜๋ฏธ๋ฅผ ๊ฐ€์งˆ ์ˆ˜๋„ ์žˆ๋‹ค. ์˜ˆ๋ฅผ ๋“ค์–ด DNA๊ฐ€ ๋™์ผํ•œ ์„œ์—ด์˜ RNA๋กœ ์ „์‚ฌ ๋˜๊ณ  The recent discovery of vast non-coding RNA-based regulatory networks that can be easily modulated by nucleic acid-based drugs has opened numerous new therapeutic possibilities. Long non-coding RNA, and natural antisense transcripts (NATs) in particular, play a significant role in networks that involve a wide variety of disease-relevant biological mechanisms such as transcription, splicing Figure 1. ASO-based gene modulation mechanisms in the nucleus. In the case of mammals, gDNA in the nucleus is transcribed to pre-mRNA. An exogenous ASO in the nucleus hybridizes A) to the 3'-most polyadenylation signal on the pre-mRNA and blocks polyadenylation at this site, thereby redirecting it to another site upstream, which upregulates gene expression 1 B) to a splice site, thereby 8V8mmvM.
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    • dna sense vs antisense